Plant Responses on Elective Gels for in vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas comosus L.)

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Berihu Mengs Gebrehiwet
1. Plant Biotechnology Lab, Jimma Agricultural Research Center, Jimma, Ethiopia; 2. Ethiopian Institute of Agricultural Research, Addis Ababa, Ethiopia

Bioprocess Engineering (Science Publishing Group) 2022
6 : 1
1-4
10.11648/j.be.20220601.11
English
Berihu Mengs Gebrehiwet. Plant Responses on Elective Gels for in vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas comosus L.). Bioprocess Engineering. Vol. 6, No. 1, 2022, pp. 1-4. doi: 10.11648/j.be.20220601.11. Share Research.
Abstract
The quest for alternative matrices for plant tissue culture is a continuing process. Agar is one of the utmost common coagulating agents in plant in vitro clonal propagation. Pure grade agar has high value price and fear of over manipulation of its resources affected searching for low cost options. Therefore, liquid medium supplement with silica gel, glass beads and sands substratum and agar in two steps of micro propagation (seedling proliferation and root induction) were investigated. The shoot multiplication in the cytokinins hormone of 2.5mg/l BA and 0.5mg/l KN supported by 7g/l agar and alternative matrices has been produced 33.1 shoots and 5.3 shoot length in sands and agar. There is none significant difference among shoot number and shoot length on the agar and sands on shoot multiplication and plant, and there is significant difference in glass beads and silica gel compared with sand/agar in the shoot multiplication, and there is highly significance in shoot length in the matrices of plant support. Pineapple root induced in auxins hormone in vitro culture of plant propagation in half strength MS media, 30g/l sugar, 1mg/l NAA and 7g/l agar. The higher root number in sands 10.6 and followed 10 in silica gel and agar supported matrices. The root length also none significant difference among means treatments in agar, glass beads and sands. However, there is a significance difference with silica gel in root length. These silica gel, glass beads and sands used as agar gels to support and aeration plantlets.
Elective Gels, Low Cost, Shoots Multiplication and Root Elongation

The quest for alternative matrices for plant tissue culture is a continuing process. Agar is one of the utmost common coagulating agents in plant in vitro clonal propagation. Pure grade agar has high value price and fear of over manipulation of its resources affected searching for low cost options. Therefore, liquid medium supplement with silica gel, glass beads and sands substratum and agar in two steps of micro propagation (seedling proliferation and root induction) were investigated. The shoot multiplication in the cytokinins hormone of 2.5mg/l BA and 0.5mg/l KN supported by 7g/l agar and alternative matrices has been produced 33.1 shoots and 5.3 shoot length in sands and agar. There is none significant difference among shoot number and shoot length on the agar and sands on shoot multiplication and plant, and there is significant difference in glass beads and silica gel compared with sand/agar in the shoot multiplication, and there is highly significance in shoot length in the matrices of plant support. Pineapple root induced in auxins hormone in vitro culture of plant propagation in half strength MS media, 30g/l sugar, 1mg/l NAA and 7g/l agar. The higher root number in sands 10.6 and followed 10 in silica gel and agar supported matrices. The root length also none significant difference among means treatments in agar, glass beads and sands. However, there is a significance difference with silica gel in root length. These silica gel, glass beads and sands used as agar gels to support and aeration plantlets.

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