Improving the Analytical Performance of PCR Assay via the First Step
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Abstract
Polymerase chain reaction (PCR) is a popular molecular biology technique that can enzymatically replicate DNA without the use of living organisms, such as E. coli or yeast. This technique allows the exponential amplification of a small number of DNA samples, while analysis becomes easier when enhanced DNA samples are applied. Developed by Dr. Kary Mullis in 1983, PCR is now a common but essential technique used in various applications for medical and biological research laboratories, including DNA cloning for sequencing, DNA-based phylogeny, functional analysis of genes, diagnosis of inherited diseases, identification of genetic fingerprints (for forensics and paternity testing), as well as detection and diagnosis of infectious diseases (e.g., COVID-19 and Monkeypox). In 1993, Dr. Mullis was awarded the Nobel Prize in Chemistry with Dr. Michael Smith for their work on PCR.
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